Descripción
| Sumario: | The Rapid Androgen Disruption Activity Reporter (RADAR) Test Guideline describes an aquatic assay that utilizes transgenic Oryzias latipes (O. latipes, Japanese medaka) eleutheroembryos at day post hatch zero, in a multi-well format to detect chemicals active on the androgen axis. The RADAR assay was designed as a screening tool to provide a short-term assay to measure the response of eleutheroembryos to chemicals potentially active on the androgen axis. Test organisms are exposed for 72 hours in six-well plates in the presence and absence of a co-treatment with 17-methyl testosterone. The assay comprises five concentrations of the test chemical. At the end of the exposure, fluorescence imaging is measured and transformed into a numerical format; the statistical analysis and data interpretation procdure enable to determine whether the test chemical is considered active or not active.
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| Notas: | OECD Guidelines for the Testing of Chemicals, Section 2 : Effects on Biotic Systems - https://doi.org/10.1787/20745761 -- OECD Guidelines for the Testing of Chemicals, Section 2 : Effects on Biotic Systems - The OECD <i>Guidelines for the Testing of Chemicals</i> is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems -- Book. |
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| Descripción Física: | 1 online resource (43 pages) |
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| ISBN: | 9789264910553 |
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