“…Emerging from the protective environment of the uterus, the newborn is exposed to a myriad of microbes, and quickly establishes a complex microbiome that shapes the infant’s biology in ways that are only now beginning to come to light. Among these exposures are a number of potential pathogens. The host responses to these pathogens in the neonatal period are unique, reflecting a developing immune system even with delivery at term. …”

“…Animals are exposed to the test substance (liquid or solid) by an appropriate route of exposure (usually by gavage using a stomach tube or a suitable intubation cannula, or by intraperitoneal injection) and are sacrificed at appropriate times after treatment. …”

“…This Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. …”

“…The cumulative percentage mortality for each exposure period is plotted against concentration on logarithmic probability paper…”

“…Induction of a dominant lethal event after exposure to a test substance (liquid, solid, vapour or gas, ...) indicates that the substance has affected germinal tissue of the test species. …”

“…At termination of the 21-day exposure period, two biomarker endpoints are measured in males and females separately, as indicators of endocrine activity of the test chemical; these endpoints are vitellogenin and secondary sexual characteristics. …”