Materias:

Materias:

Materias: “…transposon…”

Materias:

Materias:

Materias:

Tabla de Contenidos: “…DNA is the hereditary material -- Genes code for proteins -- Genes may be interrupted -- The content of the genome -- Genome sequences and gene numbers -- Clusters and repeats -- Messenger RNA -- Protein synthesis -- Using the genetic code -- Protein localization requires special signals -- Transcription -- The operon -- Regulatory RNA -- Phage strategies -- The replicon -- Extrachromosomal replicons -- Bacterial replication is connected to the cell cycle -- DNA replication -- Homologous and site-specific recombination -- Repair systems handle damage to DNA -- Transposons -- Retroviruses and retroposons -- Recombination in the immune system -- Promoters and enhancers -- Regulating eukaryotic transcription -- RNA splicing and processing -- Catalytic RNA -- Chromosomes -- Nucleosomes -- Chromatin structure is a focus for regulation -- Epigenetic effects are inherited -- Genetic engineering…”

Tabla de Contenidos: “…Apertura: primer movimiento -- Sinfonía en ADN mayor: segundo movimiento : Los secretos de la telomerasa ; Edición del ARN ; Eliminación selectiva de ADN ; En el silencio no quedarán ; Memoria transgeneracional, después de todo Lamarck tenía algo de razón ; Splicing alternativo ; El código develado de los TAL ; El juego de Tío Rico y el comienzo de la historia de los priones ; Los transposones, moléculas saltarinas -- Sinfonía genómica: tercer movimiento : Genomas pequeños ; ¿En qué se parecen una termita y un chipi-chipi? …”
Acceso restringido con credenciales UPSA

Tabla de Contenidos: “…Apertura: primer movimiento -- Sinfonía en ADN mayor: segundo movimiento : Los secretos de la telomerasa ; Edición del ARN ; Eliminación selectiva de ADN ; En el silencio no quedarán ; Memoria transgeneracional, después de todo Lamarck tenía algo de razón ; Splicing alternativo ; El código develado de los TAL ; El juego de Tío Rico y el comienzo de la historia de los priones ; Los transposones, moléculas saltarinas -- Sinfonía genómica: tercer movimiento : Genomas pequeños ; ¿En qué se parecen una termita y un chipi-chipi? …”
Acceso restringido con credenciales UPSA

Tabla de Contenidos: “…Mutagénesis con el transposón Tn5. 9.1.1. Identificación del punto de inserción del transposón Tn5 en el DNA genómico 9.1.2. …”
Enlace del recurso

Tabla de Contenidos: “…Chapter 1: Transposon-based Technologies for Insects Chapter 2: Inducible and Repressible Systems for Transgene Expression Chapter 3: Sex-, Tissue- and Stage-Specific Transgene Expression Chapter 4: RNA Interference to Modify Phenotypes in Agriculturally Important Pest and Beneficial Insects: Useful Examples and Future Challenges Chapter 5: Site-specific Recombination for Gene Locus-directed Transgene Integration and Modification Chapter 6: Receptor-Mediated Ovary Transduction of Cargo - ReMOT Control: A Comprehensive Review and Detailed Protocol for Implementation Chapter 7: Site-directed DNA Sequence Modification Using CRISPR-Cas9 Chapter 8: An Introduction to the Molecular Genetics of Gene Drives and Thoughts on Their Gradual Transition to Field Use Chapter 9: Drosophila melanogaster As a Model for Gene Drive Systems Chapter 10: Sex Ratio Manipulation Using Gene Drive for Mosquito Population Control Chapter 11: Population Modification Using Gene Drive for Reduction of Malaria Transmission Chapter 12: Modelling threshold-dependent gene drives Chapter 13: Tsetse Paratransgenesis: a Novel Strategy for Reducing the Spread of African Trypanosomiases Chapter 14: Paratransgenic Control of Chagas Disease Chapter 15: Asaia Paratrangenesis in Mosquitoes Chapter 16: Paratransgenesis in Mosquitoes and Other Insects: Microbial Ecology and Bacterial Genetic Considerations Chapter 17: Transgenic approaches for sterile insect control of dipteran livestock pests and lepidopteran crop pests Chapter 18: Honey bee genome editing Chapter 19: Progress Towards Germline Transformation of Ticks Chapter 20: Silkworm Transgenesis and its Applications Chapter 21: Tephritid Fruit Fly Transgenesis and Applications Chapter 22: Antiviral Effectors for Mosquito Transgenesis Chapter 23: Self-Limiting Insects for Pest Management Chapter 24: Public Acceptability and Stakeholder Engagement for Genetic Control Technologies Chapter 25: Regulation of Transgenic Insects Chapter 26: Economics of Transgenic Insects for Field Release Chapter 27: The Cartagena Protocol on Biosafety and the Regulation of Transboundary Movement of Living Modified Organisms Chapter 28: Risk Analysis of Transgenic Insects…”

Tabla de Contenidos: “…Crosa ... [et al.] -- Transposon mutagenesis / Frans J. De Bruijn and Silvia Rossbach -- Gene cloning and expression / Michael Bagdasarian and Mira M. …”

Tabla de Contenidos: “…Strauss -- Misalignment-mediated mutations and genetic rearrangements at repetitive DNA sequences / Susan T. Lovett -- DNA transposons: different proteins and mechanisms, but similar rearrangements / Keith M. …”

Tabla de Contenidos: “…Intro -- Title Page -- Copyright -- Contents -- Contributors -- Preface -- Acknowledgements -- 1 Transposon-Based Technologies for Insects -- 1.1 Introduction -- 1.2 Transposons Used in Insects -- 1.2.1 P elements -- 1.2.2 piggyBac -- 1.2.3 Mos1 -- 1.2.4 Minos -- 1.2.5 Hermes, Herves, hopper and hobo -- 1.2.6 Tn5 -- 1.3 Mutagenesis -- 1.4 Germline Transformation -- 1.5 Transposons as Technology Platforms -- 1.5.1 Gene expression -- 1.5.2 Cell ablation -- 1.5.3 Gene silencing -- 1.5.4 Genetic sensors -- 1.6 Hybrid Transposase Systems for Precision Integration -- 1.7 CRISPR-associated Transposases -- 1.8 Conclusion -- 2 Inducible and Repressible Systems for Transgene Expression -- 2.1 Introduction -- 2.2 Naturally Occurring Systems of Conditional Expression -- 2.2.1 Heat shock - hsp70 -- 2.2.2 Natural temperature-sensitive lethal elements and mutations -- 2.2.3 Glucose repression -- 2.2.4 Metallothionein -- 2.2.5 lac inducible systems -- 2.3 Synthetic Systems -- 2.3.1 Tetracycline-mediated expression -- 2.3.2 Dimerization -- 2.3.3 GeneSwitch -- 2.3.4 Q system -- 2.3.5 Use of Cre/loxP recombination -- 2.4 Conclusions -- 3 Sex-, Tissue- and Stage-Specific Transgene Expression -- 3.1 Introduction -- 3.2 Gene Regulation in Insects -- 3.2.1 Transcriptional control -- 3.2.2 The promoter -- 3.2.3 Enhancers and silencers -- 3.2.4 Chromatin structure and genomic position effects -- 3.3 Post-transcriptional and Translational Control -- 3.3.1 Untranslated regions and introns -- 3.3.2 Regulatory RNAs -- 3.3.3 Splicing -- 3.3.4 Translational control -- 3.4 The Basic Genetic Construct -- 3.5 Sex-Specific Gene Expression -- 3.5.1 Targeting chromosomes -- 3.5.2 Sex-specific splicing -- 3.5.3 Sex-specific promoters -- 3.6 Tissue-Specific Gene Expression -- 3.6.1 Targeting tissues relevant for parasite transmission…”

Tabla de Contenidos: “…Genes saltarines o transposones; CAPÍTULO III. LA CÉLULA: UNIDAD MORFOLÓGICA Y (...); 1. …”

Tabla de Contenidos: “…METABOLITOS, BIOPROSPECCIÓN Y LA MERCANTILIZACIÓN DE LA VIDACAPÍTULO 4 LAS BIOMOLÉCULAS Y LOS MONOCULTIVOS; LOS CARBOHIDRATOS; LOS POLISACÁRIDOS; LOS LÍPIDOS; CAPÍTULO 5 PROTEÍNAS Y ÁCIDOS NUCLEICOS: LOS MOTORES DE LA VIDA; LA PROTEÍNA; LOS ÁCIDOS NUCLEICOS; EL ADN ES LA ÚNICA MOLÉCULA CAPAZ DE REPLICARSE; LA EDICIÓN DEL ARN Y SU PAPEL EN LA EVOLUCIÓN; LOS TRANSPOSONES Y RETROGENES; LA EPIGENÉTICA; LAS MUTACIONES; LOS PRIONES; CAPÍTULO 6 ADN: DE MOLÉCULA DE LA VIDA A MATERIA PRIMA PARA LA INDUSTRIA; ¿QUÉ SON LOS TRANSGÉNICOS?…”

Tabla de Contenidos: “…EL CONTROL GENÉTICOLa hipótesis del operón; El aislamiento del represor y el operador; La búsqueda del promotor; Los transposones; El alosterismo; LA REVOLUCIÓN GENÉTICA; Arthur Kornberg; La vida se crea en un tubo de ensayo; Problemas con la DNA polimerasa; Los fragmentos de Okazaki; El nacimiento de la Ingeniería Genética; La recombinación del DNA; Los primeros contratiempos de la ingeniería genética: la reunión de Asilomar; Las nuevas técnicas de Biología Molecular; Los plásmidos; La lectura y modificación del DNA; Los anticuerpos; La huella genética…”

Tabla de Contenidos: “…Structure and Function of the Genetic Material -- Genotype and Phenotype -- DNA and Chromosomes -- The Flow of Genetic Information -- DNA Replication -- RNA and Protein Synthesis -- The Regulation of Bacterial Gene Expression -- Pre-transcriptional Control -- Post-transcriptional Control -- Changes in Genetic Material -- Mutation -- Types of Mutations -- Mutagens -- The Frequency of Mutation -- Identifying Mutants -- Identifying Chemical Carcinogens -- Genetic Transfer and Recombination -- Plasmids and Transposons -- Transformation in Bacteria -- Conjugation in Bacteria -- Transduction in Bacteria -- Genes and Evolution -- Study Outline -- Study Questions -- 9 Biotechnology and DNA Technology -- Introduction to Biotechnology -- Recombinant DNA Technology -- An Overview of Recombinant DNA Procedures -- Tools of Biotechnology -- Selection -- Mutation -- Restriction Enzymes -- Vectors -- Polymerase Chain Reaction -- Techniques of Genetic Modification -- Inserting Foreign DNA into Cells -- Obtaining DNA -- Selecting a Clone -- Making a Gene Product -- Applications of DNA Technology -- Therapeutic Applications -- Genome Projects -- Scientific Applications -- Agricultural Applications -- Safety Issues and the Ethics of Using DNA Technology -- Study Outline -- Study Questions -- PART TWO A Survey of the Microbial World -- 10 Classification of Microorganisms -- The Study of Phylogenetic Relationships -- The Three Domains -- A Phylogenetic Tree -- Classification of Organisms -- Scientific Nomenclature -- The Taxonomic Hierarchy -- Classification of Prokaryotes -- Classification of Eukaryotes -- Classification of Viruses -- Methods of Classifying and Identifying Microorganisms -- Morphological Characteristics -- Differential Staining -- Biochemical Tests -- Serology -- Phage Typing -- Fatty Acid Profiles -- Flow Cytometry -- DNA Sequencing…”

Tabla de Contenidos: “…Clinical Sampling -- Obtaining Pure Cultures -- Culture Media -- Special Culture Techniques -- Preserving Cultures -- Growth of Microbial Populations -- Generation Time -- Mathematical Considerations in Population Growth -- Phases of Microbial Population Growth -- Continuous Culture in a Chemostat -- Measuring Microbial Reproduction -- CHAPTER SUMMARY -- QUESTIONS FOR REVIEW -- CRITICAL THINKING -- CONCEPT MAPING -- 7 Microbial Genetics -- The Structure and Replication of Genomes -- The Structure of NucleicAcids -- The Structure of Prokaryotic Genomes -- The Structure of Eukaryotic Genomes -- DNA Replication -- Gene Function -- The Relationship Between Genotype and Phenotype -- The Transfer of Genetic Information -- The Events in Transcription -- Translation -- Regulation of Genetic Expression -- Mutations of Genes -- Types of Mutations -- Effects of Point Mutations -- Mutagens -- Frequency of Mutation -- DNA Repair -- Identifying Mutants, Mutagens, and Carcinogens -- Genetic Recombination and Transfer -- Horizontal Gene Transfer Among Prokaryotes -- Transposons and Transposition -- CHAPTER SUMMARY -- QUESTIONS FOR REVIEW -- CRITICAL THINKING -- CONCEPT MAPING -- 8 Recombinant DNA Technology -- The Role of Recombinant DNA Technologyin Biotechnology -- The Tools of Recombinant DNA Technology -- Mutagens -- The Use of Reverse Transcriptase to Synthesize cDNA -- Synthetic Nucleic Acids -- Restriction Enzymes -- Vectors -- Gene Libraries -- Techniques of Recombinant DNA Technology -- Multiplying DNA In Vitro: The Polymerase Chain Reaction -- Selecting a Clone of Recombinant Cells -- Separating DNA Molecules: Gel Electrophoresis and the Southern Blot -- DNA Microarrays -- Inserting DNA into Cells -- Applications of Recombinant DNA Technology -- Genetic Mapping -- Environmental Studies -- Pharmaceutical and Therapeutic Applications…”

“…The bacterial and archeal immune system CRISPR, and the eukaryotic piwi-interacting RNAs (piRNA) immune system that inhibits mobile elements in germ line cells both function by via RNA transcript/ target DNA heteroduplex base-pairing are a specific class of RNAs that protect cells from invading transposons/and or viruses. siRNAs function in plant and invertebrate immune systems and protect against viral infections…”