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Tabla de Contenidos: “…Introduction and History of the Chemistry of Nucleic Acids Therapeutics -- Antisense RNA Therapeutics: A Brief Overview -- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion -- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation -- How to Design U1 snRNA Molecules for Splicing Rescue -- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles -- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay -- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen -- Development and Use of Cellular Systems to Assess and Correct Splicing Defects -- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells -- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin -- In Vitro Delivery of PMOs in Myoblasts by Electroporation -- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts -- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy -- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro -- Eye on a Dish Models to Evaluate Splicing Modulation -- Establishment of In Vitro Brain Models for AON Delivery -- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides -- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies -- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment -- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin -- Delivery of Antisense Oligonucleotides to the Mouse Retina -- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections -- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System -- Preclinical Safety Assessment of Therapeutic Oligonucleotides -- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice -- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution -- Patent Considerations When Embarking on New Antisense Drug Programs…”

Tabla de Contenidos: “…; 3 The choice of frequence; 4 Propagation of light along the fiber; 5 Decibels; 6 Losses in optic fibers; 7 Dispersion and our attempts to prevent it; 8 Real cables; 9 Connecting optic fibers - the problems; 10 Fusion splicing; 11 Mechanical splices; 12 Connectors; 13 Couplers; 14 Light sources and detectors; 15 Testing a system; 16 System design - or will it work?…”

Tabla de Contenidos: “…Basic MathThe Order of Operations; Combining Strings; Combining Numbers and Strings; Changing the Values in Variables; Tutorial: Using Variables to Create Messages; Tutorial: Asking for Information; Arrays; Creating an Array; Accessing Items in an Array; Adding Items to an Array; Adding an item to the end of an array; Adding an item to the beginning of an array; Choosing how to add items to an array; Deleting Items from an Array; Adding and Deleting with splice(); Deleting items with splice(); Adding items with splice(); Replacing items with splice()…”

Tabla de Contenidos: “…Synchronisers: Types and UsesGang synchronisers; Synchronisers: Accessories; Sound attachments for synchronisers; Picture synchronisers; Synchroniser accessories and attachments; Joining Film: Temporary Splices; Temporary joins: tape splices; Joining Films: Permanent Splices; Welded splices; Animated Viewers; Function of the viewer in cutting; Sundry Cutting Room Equipment; Wall cuts racks; Rewind arms; Film horse; Cutting Room Accessories; Split spools and film cores; Planning Picture Sequences; Definitions; Camera viewpoint; Identifying and Printing Takes; Printing selected takes…”

Tabla de Contenidos: “…Re-Lighting and Re-Texturing -- Part 5 Editing and Audio. VSE Splicing and Operation. VSE Effects. Audio Integration -- Appendix. …”

Tabla de Contenidos: “…Liberal Subjectivity Imperiled: Norbert Wiener and Cybernetic Anxiety; 5. From Hyphen to Splice: Cybernetic Syntax in Limbo; 6. The Second Wave of Cybernetics: From Reflexivity to Self-Organization; 7. …”

“…More than a dozen septin genes in mammals, together with various splice variants displaying tissue-specific expression patterns and flexible hetero-polymeric higher-order assembly achieve an unfathomable complexity superior to the other cytoskeletal components. …”

Tabla de Contenidos: “…Apertura: primer movimiento -- Sinfonía en ADN mayor: segundo movimiento : Los secretos de la telomerasa ; Edición del ARN ; Eliminación selectiva de ADN ; En el silencio no quedarán ; Memoria transgeneracional, después de todo Lamarck tenía algo de razón ; Splicing alternativo ; El código develado de los TAL ; El juego de Tío Rico y el comienzo de la historia de los priones ; Los transposones, moléculas saltarinas -- Sinfonía genómica: tercer movimiento : Genomas pequeños ; ¿En qué se parecen una termita y un chipi-chipi? …”
Acceso restringido con credenciales UPSA