Tabla de Contenidos: “…. -- There's more... -- The missing link -- Getting started -- How to do it... -- How it works... -- There's more... -- Searching high and low -- Getting started -- How to do it... -- How it works... -- There's more... -- Chapter 10: Exploring Windows Forensic Artifacts Recipes - Part II -- Introduction -- Parsing prefetch files -- Getting started -- How to do it... -- How it works... -- There's more... -- A series of fortunate events -- Getting started -- How to do it... -- How it works... -- There's more... -- Indexing internet history -- Getting started -- How to do it... -- How it works... -- There's more... -- Shadow of a former self -- Getting started -- How to do it... -- How it works... -- There's more... -- Dissecting the SRUM database -- Getting started -- How to do it... -- How it works... -- There's more... -- Conclusion -- Index…”

Tabla de Contenidos: “…Little Endian -- Pros of Little Endian -- About Memory Addresses -- Loading a Register with an Address -- PC Relative Addressing -- Loading Data from Memory -- Combining Loading Addresses and Memory -- Storing a Register -- Optimizing Through Relaxing -- Converting to Uppercase -- Summary -- Exercises -- Chapter 6: Functions and the Stack -- About Stacks -- Jump and Link -- Nesting Function Calls -- Function Parameters and Return Values -- Managing the Registers -- Summary of the Function Call Algorithm -- Uppercase Revisited -- Stack Frames -- Stack Frame Example -- Defining Symbols -- Macros -- Include Directive -- Macro Definition -- Labels -- Why Macros? …”

Tabla de Contenidos: “…Deep diving into the lifecycle of a real app -- Adding conditional rendering to the sidebar -- Writing the logic to handle the sidebar states -- Save and read the user preference in localStorage -- Summary -- Chapter 10: Handling Routing with Vue Router -- Technical requirements -- Introducing vue-router -- Learning about vue-router configuration -- Creating our first view -- Navigating between routes -- Using the &lt -- router-link&gt -- component -- Programmatic navigation -- Dynamic route matching -- Creating a user profile page -- Creating a user profile route -- Adding navigation using the route name -- Reading route parameters in a route component -- Learning about nested routes -- Applying nested routes to the user -- Reusing routes with alias and redirect -- Summary -- Chapter 11: Managing Your Application's State with Pinia -- Technical requirements -- When to use state management -- Learning about the structure of a Pinia store -- Centralized sidebar state management with Pinia -- Creating our first store -- Creating a post store with Pinia -- Implementing an Add Post action -- Summary -- Chapter 12: Achieving Client-Side Validation with VeeValidate -- Technical requirements -- Understanding forms -- Wrapping your form within the &lt -- form&gt -- element -- Do not forget labels -- There is more than just type="text" -- Setting your form to be autocompleted -- Two-way binding with v-model -- Controlling your form using VeeValidate -- Defining your form validation with VeeValidate -- Using VeeValidate rules -- Summary -- Part 4: Conclusion and Further Resources -- Chapter 13: Unveiling Application Issues with the Vue Devtools -- Getting familiar with the Vue Devtools -- Debugging components in your Vue Devtools -- Use cases for the Vue Devtools information panel -- Deep dive into the Vue Devtools Timeline tab…”

Tabla de Contenidos: “…Using Utility Filters -- Using Non-Corrective Filters -- Using Other Filters -- Lesson Review -- Lesson 9 Creating Dynamic Effects -- Keyframing Basics -- Keyframing in the Canvas -- Modifying the Path -- Smoothing Your Path -- Moving the Path -- Deleting Keyframes -- Controlling Acceleration -- Keyframing in the Viewer -- Smoothing Keyframes -- Animating Filters -- Keyframing in the Timeline -- Displaying Keyframe Bars -- Customizing Keyframe Bars -- Editing Keyframe Graphs in the Timeline -- Bonus Exercise -- Lesson Review -- Lesson 10 Nesting Sequences -- Understanding Nested Sequences -- Basic Nesting -- Understanding Live Links -- Applying Effects to Multiple Clips -- Applying Complex Geometric Settings -- Applying Motion Parameters -- Add a Background Element -- Adjusting the Anchor Point -- Changing the Render Order -- Reordering Motion Effects -- Mixing Sequence Sizes -- Using Nesting as an Editing Tool -- Marking a Sequence with In and Out Points -- Editing Within the Nested Sequence -- Using Pre-Trimmed Subsequences -- Editing the Content of a Subsequence -- Lesson Review -- Lesson 11 Variable Speed -- Constant Speed Changes -- Maintaining Sequence Length -- Scaling Attributes -- Displaying Speed Indicators -- Variable Speed Changes -- Ramping Constant Speed Effects -- Creating Speed Segments -- Smoothing Speed Segments -- Custom Speed Keyframe Curves -- Dragging Speed Keyframes -- Creating a Temporary Freeze -- Using the Speed Tool -- Removing Speed Effects -- Copying and Saving Speed Effects -- Lesson Review -- Cameo: RED Baron-Brian Gonosey -- Finishing Techniques -- Lesson 12 The Big Finish -- Widescreen Workflows -- Converting 4:3 Footage to Widescreen -- Converting Widescreen Footage to 4:3 -- More Letterboxing Techniques -- Managing Your Media -- Consolidating Media -- Moving Media -- Trimming Sequences in Media Manager…”

Tabla de Contenidos: “…-- Performance of Branch Instructions -- Summary -- Exercises -- Chapter 6: Thanks for the Memories -- How to Define Memory Contents -- How to Align Data -- How to Load a Register -- How to Load a Register with an Address -- How to Build the Address Directly -- PC Relative Addressing -- How to Load Data from Memory -- Optimizing Small Read-Only Data Access -- Indexing Through Memory -- How to Store a Register -- How to Convert to Uppercase -- How to Load and Store Multiple Registers -- Summary -- Exercises -- Chapter 7: How to Call Functions and Use the Stack -- About Stacks on the RP2040 -- How to Branch with Link -- About Nesting Function Calls -- About Function Parameters and Return Values -- How to Manage the Registers -- Summary of the Function Call Algorithm -- More on the Branch Instructions -- About the X Factor -- Uppercase Revisited -- About Stack Frames -- Stack Frame Example -- How to Define Symbols -- How to Create Macros -- About Include Directive -- How to Define a Macro -- About Labels -- Why Macros? …”

Tabla de Contenidos: “…Menu Reference -- Safety Menu -- Obstacle Avoidance Action (Mini 3 Pro Only) -- Disable Sideways Flight (Mini 3 Pro Only) -- Display Radar Map (Mini 3 Pro Only) -- Max Altitude/Max Distance -- Auto RTH Altitude -- Update Home Point -- Compass Calibration -- IMU Normal -- Battery Info -- Unlock GEO Zone -- Find My Drone -- Advanced Safety Settings -- Control Menu -- Units -- Subject Scanning (Mini 3 Pro Only) -- Gain &amp -- Expo Tuning -- Gimbal Mode -- Gimbal Calibration -- Recenter Gimbal -- Stick Mode -- Button Customization -- RC Calibration -- Flight Tutorial -- Re-pair to Aircraft (Link) -- Camera Menu -- Format (Mini 3 Pro Only) -- Aspect Ratio (Mini 3 Pro Only) -- Anti-Flicker (Mini 3 Pro Only) -- Histogram/Peaking Level/Overexposure Warning -- Gridlines -- White Balance -- Style (Mini 3 Pro Only) -- Format -- USB Mode (Mini 3 Pro Only) -- Auto Sync HD Photos (Mini 3 Only) -- Cache When Recording -- Max Video Cache Capacity -- Reset Camera Parameters -- Transmission Menu -- Live-Streaming Platforms -- HDMI Output (Mini 3 Pro Only) -- Frequency/Channel Mode -- About Menu -- DJI Fly Main Screen -- Next Up -- Index…”

Tabla de Contenidos: “…-- Random oversampling -- Problems with random oversampling -- SMOTE -- How SMOTE works -- Problems with SMOTE -- SMOTE variants -- Borderline-SMOTE -- ADASYN -- Working of ADASYN -- Categorical features and SMOTE variants (SMOTE-NC and SMOTEN) -- Model performance comparison of various oversampling methods -- Guidance for using various oversampling techniques -- When to avoid oversampling -- Oversampling in multi-class classification -- Summary -- Exercises -- References -- Chapter 3: Undersampling Methods -- Technical requirements -- Introducing undersampling -- When to avoid undersampling the majority class -- Fixed versus cleaning undersampling -- Undersampling approaches -- Removing examples uniformly -- Random UnderSampling -- ClusterCentroids -- Strategies for removing noisy observations -- ENN, RENN, and AllKNN -- Tomek links -- Neighborhood Cleaning Rule -- Instance hardness threshold -- Strategies for removing easy observations -- Condensed Nearest Neighbors -- One-sided selection…”

Tabla de Contenidos: “…On Publishing Cryptographic Algorithms -- Two More Open-Source Fallacies -- The Microsoft Fallacy -- The Java Fallacy -- An Example: GNU Mailman Security -- More Evidence: Trojan Horses -- To Open Source or Not to Open Source -- Another Security Lesson from Buffer Overflows -- Beating the Drum -- Conclusion -- 5 Guiding Principles for Software Security -- Principle 1: Secure the Weakest Link -- Principle 2: Practice Defense in Depth -- Principle 3: Fail Securely -- Principle 4: Follow the Principle of Least Privilege -- Principle 5: Compartmentalize -- Principle 6: Keep It Simple -- Principle 7: Promote Privacy -- Principle 8: Remember That Hiding Secrets Is Hard -- Principle 9: Be Reluctant to Trust -- Principle 10: Use Your Community Resources -- Conclusion -- 6 Auditing Software -- Architectural Security Analysis -- Attack Trees -- Reporting Analysis Findings -- Implementation Security Analysis -- Auditing Source Code -- Source-level Security Auditing Tools -- Using RATS in an Analysis -- The Effectiveness of Security Scanning of Software -- Conclusion -- 7 Buffer Overflows -- What Is a Buffer Overflow? …”

Tabla de Contenidos: “…-- Extending an Extension Point -- Examining the Sample Extension -- Modifying the Sample Extension -- Extension UI Configuration -- Configuration File Conventions -- Configuration UI Rendering -- Interaction Between UI and Model -- UI Validation -- Global UI Configuration -- Summary -- Part III: The Hudson Lifestyle -- Chapter 9: Advanced Plugin Development -- Extending Various Aspects of Hudson Dashboards -- Creating a Custom Rendered Jobs Status View -- Using the Custom Rendered View -- Adding a Custom Column to the Default View -- Adding an Action to the Action Panel of the Main Dashboard -- Custom Decoration of Hudson Pages -- Extending Various Aspects of a Hudson Job -- Adding a Custom Notifier to a Job -- Adding a Custom Link to a Job Dashboard -- Creating a Custom Build Wrapper -- Summary -- Chapter 10: Hudson Best Practices -- Manage the Hudson Resources Effectively -- Tune Memory -- Restrict Job History Depth -- Monitor the Disk Space -- Put Your Hudson Behind a Web Proxy -- Do Not Use Your Hudson as a File Server -- Periodically Back Up Your Hudson Contents -- Set Up a Fail-Safe Mode for Your Hudson -- Redundant Hudson Server Setup -- Monitoring Hudson Setup -- Server Switch on Failure -- Redirecting HTTP Traffic to a Redundant Server -- Scale Up the Security of Your Hudson -- Restrict Job Access to Certain Users -- Add Roles to the Authorization Matrix -- Upgrade Your Hudson Diligently -- Understand the Hudson Versioning Scheme -- Upgrade in a Sandbox First -- Summary -- Part IV: Appendixes -- Appendix A: Widely Used Hudson Plugins -- Appendix B: Personal Hudson Instance -- Hudson-as-Valet -- Optimal Hudson-as-Valet Setup -- Hudson for Work Area Maintenance -- The Open-Source Liaison Role -- The Committer Role -- Hudson as General-Purpose Scheduler…”

Tabla de Contenidos: “…5.7 Concept Metaphors and Analogies -- 5.8 Role-Play Ideation -- 5.9 Ideation Game -- 5.10 Puppet Scenario -- 5.11 Behavioral Prototype -- 5.12 Concept Prototype -- 5.13 Concept Sketch -- 5.14 Concept Scenarios -- 5.15 Concept Sorting -- 5.16 Concept Grouping Matrix -- 5.17 Concept Catalog -- Mode 6: Frame Solutions -- Frame Solutions: Mindsets -- Conceiving Holistic Solutions -- Conceiving Options -- Making Value Judgments -- Envisioning Scenarios -- Structuring Solutions -- Frame Solutions: Methods -- 6.1 Morphological Synthesis -- 6.2 Concept Evaluation -- 6.3 Prescriptive Value Web -- 6.4 Concept-Linking Map -- 6.5 Foresight Scenario -- 6.6 Solution Diagramming -- 6.7 Solution Storyboard -- 6.8 Solution Enactment -- 6.9 Solution Prototype -- 6.10 Solution Evaluation -- 6.11 Solution Roadmap -- 6.12 Solution Database -- 6.13 Synthesis Workshop -- Mode 7: Realize Offerings -- Realize Offerings: Mindsets -- Reiterating Prototypes -- Evaluating in Reality -- Defining Strategies -- Implementing in Reality -- Communicating Vision -- Realize Offerings: Methods -- 7.1 Strategy Roadmap -- 7.2 Platform Plan -- 7.3 Strategy Plan Workshop -- 7.4 Pilot Development and Testing -- 7.5 Implementation Plan -- 7.6 Competencies Plan -- 7.7 Team Formation Plan -- 7.8 Vision Statement -- 7.9 Innovation Brief -- Credits for Example Projects -- Index -- EULA…”

Tabla de Contenidos: “…Die Erinnerungsfunktion -- Gemeinsam genutzte Funktionen und Befehle -- Die wichtigsten Neuerungen bei den gemeinsam genutzten Funktionen -- Dokumente illustrieren -- Bilder und andere Grafikdateien -- Onlinegrafiken -- Bilder bearbeiten -- Geometrische Formen -- SmartArt -- Objekte aus anderen Programmen -- WordArt -- Links -- Dokumente drucken -- Übersicht zu den Druckaufgaben -- Excel: die Einstellungen für einzelne Blätter festlegen -- Word: Seriendruck -- Briefumschläge und Etiketten erstellen -- Druckereinstellungen ändern -- Arbeiten in Teams -- Dokumente kommentieren -- Dokumente korrigieren (lassen) -- Korrekturen anzeigen -- Änderungen prüfen -- Das Dateiformat ändern -- Dokumente zur gemeinsamen Bearbeitung zur Verfügung stellen -- Mit einer Office Web App arbeiten -- Dokumente versenden -- Stichwortverzeichnis…”

Tabla de Contenidos: “…Images and Graphics -- Comparing Vectors and Bitmaps -- Scalable Vector Graphics -- Anatomy of an SVG Image -- Linked SVG Files -- SVG Sprites -- SVG Sprites with Fragments -- Embedded SVG -- SVG Filters -- The Convergence of SVG and CSS -- A Drawback of SVG -- The canvas Element -- Image Manipulation -- WebGL -- When to Choose SVG or Canvas -- Summary -- H. …”

Tabla de Contenidos: “…Adding graphics from other programs -- Comparing vector and bitmap graphics -- Managing links to imported files -- Updating revised graphics -- Adjusting display quality -- Working with clipping paths -- Working with alpha channels -- Importing native Adobe graphics files -- Using a library to manage objects -- Using Adobe Bridge to import graphics -- Exploring on your own -- Review questions and answers -- 11 CREATING TABLES -- Lesson overview -- Getting started -- Converting text to a table -- Formatting a table -- Creating a header row -- Adding graphics to table cells -- Creating and applying table and cell styles -- Exploring on your own -- Review questions and answers -- 12 WORKING WITH TRANSPARENCY -- Lesson overview -- Getting started -- Importing and colorizing a grayscale image -- Applying transparency settings -- Adjusting transparency settings for EPS images -- Adding transparency effects -- Importing and adjusting Illustrator files that use transparency -- Applying transparency settings to text -- Working with effects -- Exploring on your own -- Review questions and answers -- 13 OUTPUT AND EXPORTING -- Lesson overview -- Getting started -- Preflighting files -- Packaging files -- Creating an Adobe PDF proof -- Previewing separations -- Previewing how transparency effects will be flattened -- Previewing the page -- Printing a laser or inkjet proof -- Using the Ink Manager -- Exploring on your own -- Review questions and answers -- 14 CREATING RICH INTERACTIVE DOCUMENTS -- Lesson overview -- Getting started -- Setting up an online document -- Adding buttons, page transitions, and hyperlinks -- Exporting as Flash Player (SWF) -- Exporting as Adobe PDF (Interactive) -- Converting a print document for online use -- Adding advanced interactivity, animation, and video -- Exploring on your own -- Review questions and answers…”

Tabla de Contenidos: “…Managing packages -- Dependencies -- Bootstrap dependency -- A better Hello World -- Modules -- Components -- Handling events -- Data binding -- One way -- Two way -- Services -- Routes -- routerLinkActive -- Building a project -- Setup and run sample -- Introduction to PrimeNG -- Summary -- Chapter 10: Angular Forms -- Two approaches to forms -- Template-driven forms -- Reactive forms -- Understanding forms with an example -- Building custom forms with validations -- Checking the state of a form -- Forms with NgModel -- Reactive forms -- Setting up forms with FormBuilder -- Adding validations -- Creating a custom validator -- Grouping controls -- Setting and patching values -- Handling forms in Angular -- Gaining more control with reactive forms -- Summary -- Chapter 11: Building a Real-World Application -- Building an Issue Management System frontend -- Setup -- Structuring the project -- Working independently of the backend -- Data models -- Securing the application -- Issue lists and details -- Rendering data with templates -- Injectable service -- Issue creation and updates -- Reactive forms -- Validation -- Issue comments -- Chatting on an issue -- Production-ready build -- Summary -- Chapter 12: Connecting Angular to Java EE Microservices -- Integration with microservices -- Docker - focusing made easy -- Verifying the running services -- Cross Origin Resource Sharing (CORS) -- JSON communication -- Observables in Angular -- Dynamic updates using Observables -- Going to production -- Deploying on the cloud using AWS -- Launching the Amazon EC2 instance -- Installing Docker on Amazon EC2 -- Docker release -- Summary -- Chapter 13: Testing Java EE Services -- Testing strategies for microservices -- Understanding the testing pyramid -- Unit testing -- Integration testing -- End-End testing -- Microservice testing challenges -- Contract testing…”

Tabla de Contenidos: “…Design How can the PMO Link Strategy with Execution? Selecting, Prioritizing and Balancing: Portfolio Management Resource Management: Is it Possible to Defy the Laws of Physics? …”

Tabla de Contenidos: “…Replacing the songList array with an XML instance -- Loading an external playlist using the URLLoader class -- Responding to COMPLETE and IO_ERROR events -- Moving the event listeners into the xmlLoaded() function -- Updating the chooseSong() function -- Creating hyperlinks using XML data -- Navigating through the song list -- Some suggestions to try on your own -- 11 USING ACTIONSCRIPT AND COMPONENTS TO CONTROL VIDEO -- Lesson overview -- Examining the Lesson11 folder contents -- Adding the FLVPlayback component -- Setting FLVPlayback properties in Flash -- Adding ActionScript control of FLVPlayback properties -- Working with color -- Using cue points with Flash video -- Adding the FLVPlaybackCaptioning component -- Playing multiple video files from an XML playlist -- Using the full-screen publish settings -- Some suggestions to try on your own -- 12 DELVING DEEPER INTO GRAPHICS AND ANIMATION WITH ACTIONSCRIPT -- Lesson overview -- Inverse kinematics in Flash CS5 -- Examining the starting files -- Working with IK animation in ActionScript -- Creating Sound and SoundChannel instances -- Accessing the user's webcam or video camera using ActionScript -- Using the Bitmap and BitmapData classes -- Examining the Pixel Bender Toolkit -- Suggestions to try on your own -- 13 PRINTING AND SENDING EMAIL WITH ACTIONSCRIPT 3.0 -- Lesson overview -- Examining the starting file -- Adding a simple email link -- Sending email from Flash -- Adding printing capabilities with the PrintJob class -- Some suggestions to try on your own -- 14 CREATING ADOBE AIR APPLICATIONS WITH FLASH AND ACTIONSCRIPT -- Lesson overview -- Using AIR-specific ActionScript -- Specifying Flash publish settings for an AIR project -- Creating an AIR application -- Examining the starting file -- Controlling printing with AIR -- Listening for drag events -- Some suggestions to try on your own…”

Tabla de Contenidos: “…Printers in the classroom: Teaching design, engineering, and art ; Hands-on history ; William Hand, Jr. boat hull ; Herreshoff cleat ; Reactions to the 3D prints ; Learning through re-creating history ; The special-needs student ; After-school activities ; Robotics clubs and teams ; DIY girls ; Young maker programs ; Career tech ed ; Early-adopter experiences -- Scientific visualization: Visualizing molecular biology ; Model accuracy considerations ; Example: 3D-printed models of six-helix DNA bundles ; Visualizing mathematical abstractions ; Parabola math manipulative ; Surfaces of revolution ; Sinusoids ; General surface modeling ; Other scientific uses of 3D printing -- Futures: Technology trends ; Extreme users ; Improving the user experience ; Faster printing ; Filament ; Emerging 3D-printing applications ; Printing food ; 3D printing in medicine ; The developing world ; The business of 3D printing ; Printer patent issues ; Hardware as a service -- Appendix A: typical printer settings: Slic3r typical settings ; Cura settings difference -- Appendix B: links and resources: A brief history of 3D printing ; The desktop 3D printer ; Open source ; Making a 3D model ; Slicing a 3D model ; Driving your printer: G-Code ; Material considerations ; Case studies ; Moving to metal ; Large prints and post-processing ; Troubleshooting ; Printers in the classroom ; Scientific visualization ; Futures ; Focusing on 3D printing…”

Tabla de Contenidos: “…StraubInklusions-, Exklusions- und Behinderungserfahrungen Studierenderzwischen Familie, Schule und Hochschule Eine Narrationsanalysebiographischer Erzählungen zu Barrieren in tertiären Bildungsräumen -- Ulrike Barth und Angelika WiehlWahrnehmungsvignetten als Grundlage einer pädagogischenHaltungsentwicklung -- Robert Langnickel und Pierre-Carl LinkInklusive pädagogische Räume Psychoanalytische Pädagogik im Feldeiner Inklusiven Bildungspraxis -- IV Raumproduktion und RaumgestaltungJens Geldner-Belli und Steffen WittigDie Universität als demokratischer, pädagogischer und inklusiver Raum?…”

Tabla de Contenidos: “…-- 8.5 Significance of Iranian Internal Waters for the Passage Regime in the Strait of Hormuz -- 9 Tensions in and over the Taiwan Strait in 2021 -- 9.1 Legal and Geographical Characteristics of the Taiwan Strait -- 9.2 Navigation in the Taiwan Strait in the Light of Recent Developments in China’s Legislation -- 9.3 Geopolitical Tensions in the Taiwan Strait and Intrusions of Taiwan’s Air Defence Identification Zone -- 10 Discriminatory Navigational Restrictions in the Kerch Strait in Respect of Foreign Commercial Ships -- 10.1 The Significance of the Kerch Strait for Commerce -- 10.2 Restrictions on Foreign Commercial Ships’ Navigation through the Kerch Strait -- 11 Discriminatory Prohibition of the Right of Innocent Passage of a Commercial Ship  The Vironia Incident in the Gulf of Finland -- 11.1 Right of Innocent Passage in the Eastern Gulf of Finland from 1920s to 2000 -- 11.2 The Russian Federation’s Maritime Zones in the Gulf of Finland -- 11.3 The Vironia Incident in the Gulf of Finland and Its Aftermath -- 11.4 Potential Legal Basis of the Russian Federation’s Permit-Based Passage Regime in the Gulf of Finland -- part 4 -- Major Maritime Industrial Projects, Piracy, and Unidentified Soldiers -- 12 The Nord Stream Project and Estonian-Russian Incidents in the Viro Strait -- 12.1 Link between Industrial Projects and Maritime Security -- 12.2 The Significance of the Viro Strait’s eez Corridor for the Nord Stream Project -- 12.3 Marine Scientific Research in the Context of Seabed Studies on the Pipeline Route -- 12.4 The Incident between the Estonian Coast Guard and Russian Research Vessels in the Viro Strait’s eez Corridor -- 12.5 Permit-Based Marine Scientific Research in an eez: Estonia’s Decision to Deny Seabed Surveys -- 13 Countering the Threat of ‘Little Green Men’ in the Åland Strait -- 13.1 Geopolitical Characteristics of the Åland Strait and Preparations to Counter Unidentified Soldiers on the Åland Islands -- 13.2 Legal Regime of the Åland Strait -- 14 Threats of Piracy in the Straits of Malacca, Sunda, Lombok -- 14.1 Legal and Geopolitical Characteristics -- 14.2 Threats of Piracy in Indonesia and the Straits of Malacca and Singapore -- part 5 -- Concluding Observations on the Implications of Hybrid Threats for Maritime Security Law -- 15 A Need for a New Legal Framework on Hybrid Naval Warfare? …”

Tabla de Contenidos: “…Summary -- Introduction -- 1.1 Protein aggregation and amyloidoses -- 1.1.1 Mechanisms of amyloid fibril formation -- 1.1.2 Oligomer structural polymorphism: fibrillar and prefibrillar oligomers -- 1.1.3 Oligomer toxicity: common mechanism of pathogenesis -- 1.1.4 Amyloid formation is an inherent property of polypeptide chains: functional amyloid and disease unreleated amyloid -- 1.2 HypF-N: model protein of amyloid aggregation unrelated to disease -- 1.2.1 Function, structure and aggregation of HypF-N -- 1.2.2 HypF-N protofibrils interact with cell membranes originating a cytotoxic cascade -- 1.2.3 A causative link between the structure of HypF-N oligomers and their ability to cause cellular dysfunction -- 1.3 Alzheimer's disease -- 1.3.1 The Alzheimer phenotype -- 1.3.2 The elaborate processing of APP -- 1.3.3 The genetics of Alzheimer's disease -- 1.3.4 Peripheral cells as a tool to identify and test hypotheses on AD pathophysiology -- 1.3.5 Adult neurogenesis and stem cell technology for AD -- 1.4 Cholesterol and gangliosides in the central nervous system (CNS) -- 1.4.1 Brain cholesterol metabolism -- 1.4.2 Ganglioside metabolism -- 1.4.3 Lipid rafts -- 1.4.4 Role of cholesterol in AD -- 1.4.5 Role of gangliosides in AD -- 1.5 Aim of the study -- Materials & Methods -- 2.1 Materials -- 2.1.1 Chemicals -- 2.1.2 Fluorescent probes -- 2.1.3 Peptides and aggregation protocols -- 2.2 Cell cultures -- 2.3 Methods -- 2.3.1 Separation processes -- 2.3.2 Differentiation of human mesenchymal stromal cells -- 2.3.3 Modulation of membrane cholesterol levels -- 2.3.4 Modulation of membrane GM1 levels -- 2.3.5 Cholesterol content measurements -- 2.3.6 GM1 content measurements -- 2.3.7 Cell exposure to peptide aggregates -- 2.3.8 Analysis of aggregate interaction with the cells -- 2.3.9 Analysis of aggregate interaction with GM1 -- 2.3.10 Analysis of aggregate internalisation -- 2.3.11 Analysis of membrane permeability -- 2.3.12 Analysis of cytosolic Ca2+ dyshomeostasis -- 2.3.13 Evaluation of ROS production -- 2.3.14 Analysis of lipid peroxidation -- 2.3.15 Cytotoxicity assay and cell death analysis: apoptotic and necrotic markers -- 2.3.16 Steady-state fluorescence anisotropy -- 2.3.17 Atomic force microscopy (AFM) -- 2.3.18 Measurements of the fluorescence intensities -- 2.3.19 Statistical analysis -- Results -- 3.1 Results I -- 3.1.1 A protective role for lipid raft cholesterol against amyloidinduced membrane damage in human neuroblastoma cells -- 3.1.2 Aß42 oligomer binding to the cell surface and its cytotoxic effect are modulated by membrane cholesterol content -- 3.1.3 Aß42 oligomers colocalize with lipid rafts -- 3.1.4 Isolation and characterization of DRMs -- 3.1.5 Effects of ADDLs on lipid raft structural order -- 3.1.6 AFM imaging of supported DRMs purified from cells exposed to ADDLs -- 3.2 Results II -- 3.2.1 Lipid rafts mediate amyloid-induced calcium dyshomeostasis and oxidative stress in Alzheimer's disease -- 3.2.2 Lipid rafts are primary interaction sites for Aß42 oligomers at the plasma membrane -- 3.2.3 Cholesterol and GM1 mediate Aß42 accumulation at the plasma membrane -- 3.2.4 Cholesterol and GM1 mediate Ca2+ dyshomeostasis and extensive membrane permeabilization induced by Aß42 oligomers -- 3.2.5 GM1 modulates lipid peroxidation and cytotoxicity induced by Aß42 oligomers -- 3.2.6 GM1 mediates Aß42-induced Ca2+ dyshomeostasis, lipid peroxidation and cytotoxicity in rat cortical neurons -- 3.3 Results III -- 3.3.1 Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers -- 3.3.2 Membrane cholesterol content modulates oligomer cytotoxicity -- 3.3.3 Membrane cholesterol modulates oligomer-induced alteration of intracellular Ca2+ homeostasis and ROS levels -- 3.3.4 Cholesterol levels modulate membrane permeability to the oligomers -- 3.3.5 Membrane GM1 affects the cytotoxic and permeabilizing effects of HypF-N oligomers -- 3.3.6 GM1, rather than cholesterol, plays a dominant role in oligomer cytotoxicity and membrane permeability -- 3.4 Results IV -- 3.4.1 Neuronal differentiation of human mesenchymal stromal cells increases their resistance to Aß42 aggregate toxicity -- 3.4.2 Neuronal differentiation of hMSCs results in reduced levels of membrane GM1 -- 3.4.3 Neuronal differentiation of hMSCs reduces the interaction of Aß42 oligomers with the cell surface -- 3.4.4 Neuronal differentiation of hMSCs reduces Aß42 oligomerinduced intracellular Ca2+ dyshomeostasis and oxidative stress -- 3.4.5 Neuronal differentiation of hMSCs increases cell resistance to Aß42 aggregates Discussion -- 4.1 A protective role for lipid raft cholesterol against amyloid-induced membrane damage in human neuroblastoma cells -- 4.2 Lipid rafts mediate amyloid-induced calcium dyshomeostasis and membrane permeabilization in Alzheimer's fibroblasts -- 4.3 Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers -- 4.4 Neuronal differentiation of human mesenchymal stromal cells increases their resistance to Aß42 aggregate toxicity -- 4.5 Concluding remarks -- Abbreviations -- References…”