Principles of gene manipulation and genomics

Detalles Bibliográficos
Autor principal: Primrose, S. B. (-)
Otros Autores: Twyman, Richard M.
Formato: Libro
Idioma:Inglés
Publicado: Malden, MA ; Oxford : Blackwell Pub 2006
Edición:7th ed
Materias:
Ver en Universidad de Navarra:https://unika.unav.edu/discovery/fulldisplay?docid=alma991004068699708016&context=L&vid=34UNAV_INST:VU1&search_scope=34UNAV_TODO&tab=34UNAV_TODO&lang=es
Tabla de Contenidos:
  • Gene manipulation in the post-genomics era
  • Basic techniques
  • Cutting and joining DNA molecules
  • Basic biology of plasmid and phage vectors
  • Cosmids, phasmids, and other advanced vectors
  • Gene cloning strategies
  • Sequencing genes and short stretches of DNA
  • Changing genes : site-directed mutagenesis and protein engineering
  • Bioinformatics
  • Cloning in bacteria other than Escherichia coli
  • Cloning in Saccharomyces cerevisiaea and other fungi
  • Gene transfer to animal cells
  • Genetic manipulation of animals
  • Gene transfer to plants
  • Advanced transgenic technology
  • The organization and structure of genomes
  • Mapping and sequencing genomes
  • Comparative genomics
  • Large-scale mutagenesis and interference
  • Analysis of the transcriptome
  • Proteomics I : expression analysis and characterization of proteins
  • Proteomics II : analysis of protein structures
  • Proteomics III : protein interactions
  • Metabolomics and global biochemical networks
  • Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci
  • Applications of recombinant DNA technology.