BioBuilder

Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular...

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Detalles Bibliográficos
Otros Autores: Kuldell, Natalie, author (author), Bernstein, Rachel, author, Ingram, Karen, author, Hart, Kathryn M., author
Formato: Libro electrónico
Idioma:Inglés
Publicado: Sebastopol, California : O'Reilly Media, Inc 2015.
Edición:First edition
Materias:
Ver en Biblioteca Universitat Ramon Llull:https://discovery.url.edu/permalink/34CSUC_URL/1im36ta/alma991009629937506719
Tabla de Contenidos:
  • ""Copyright""; ""Table of Contents""; ""Preface""; ""Who Should Read This Book""; ""Why We Wrote This Book""; ""A Word on Synthetic Biology Today""; ""Navigating This Book""; ""Online Resources""; ""Safari® Books Online""; ""How to Contact Us""; ""Acknowledgments""; ""Chapter 1. Fundamentals of Synthetic Biology""; ""What Is Synthetic Biology?""; ""Why Synthetic Biology?""; ""Synthetic Biology in Context""; ""Introduction to Engineering and Design""; """Traditional" Engineering Solutions""; ""Engineering Toolkits""; ""The Synthetic Biology Toolkit""; ""The Molecular Biology Toolkit""
  • ""The Toolkit Expanded for Synthetic Biology""""Wrap-Up""; ""Additional Reading and Resources""; ""Chapter 2. Fundamentals of Biodesign""; ""Illustrating a Top-Down Design Approach""; ""From Planning Vacations to Biodesign""; ""From Design to Implementation""; ""Biodesign Process Overview""; ""Identify an Area and Challenge""; ""Brainstorm Solutions to the Challenge""; ""Decide on an Approach""; ""Specify Your System with Devices, Parts, and DNA""; ""What's Next?""; ""Additional Reading and Resources""; ""Chapter 3. Fundamentals of DNA Engineering""; ""Framing the Discussion""
  • ""Standardization of Parts and Measurements""""What Is Standardization?""; ""Competing Standards""; ""How Standards Are Established""; ""DNA Engineering in Practice""; ""DNA Assembly Basics""; ""Applying the Basics of DNA Assembly""; ""What's Next?""; ""Additional Reading and Resources""; ""Chapter 4. Fundamentals of Bioethics""; ""What Makes "Good Work"?""; ""Regulating for Ethical Research""; ""Scientific Advances Raise Ethical Questions""; ""Public Response""; ""Three Synthetic Biology Case Studies""; ""Grow-Your-Own Glowing Plants""; """Creating" Genomes""
  • ""Biosynthetic Drugs and the Economics of Public Benefit""""Group Bioethics Activities""; ""Bioethics Involves a Decision-Making Process""; ""Stakeholder Activity""; ""Additional Reading and Resources""; ""Chapter 5. Introduction to the BioBuilder Labs""; ""Biological Engineering with Synthetic Biology's Toolkit""; ""Chapter 6. Eau That Smell""; ""Inspiration from the Eau d'coli iGEM Project""; ""Identify a Challenge""; ""Brainstorm Solutions""; ""System-Level Design""; ""Device-Level Design""; ""System-Level Design, Revised""; ""Device-Level Design, Revised""; ""Parts-Level Design""
  • ""Final Device and System-Level Edits""""Additional Reading and Resources""; ""Eau That Smell Lab""; ""Design Choices""; ""Experimental Question""; ""Getting Started""; ""Lab Protocol""; ""Chapter 7. iTune Device""; ""Modularity""; ""Insulation""; ""Principles of Measurement""; ""What's Normally Measured""; ""Making and Reporting Measurements for Synthetic Biology""; ""Foundational Concepts for the iTune Device Lab""; ""Promoters and RBSs""; ""The Lac Operon""; ""Additional Reading and Resources""; ""iTune Device Lab""; ""Design Choices""; ""Experimental Question""; ""Getting Started""
  • ""Lab Protocol""