Biochemistry laboratory manual for undergraduates an inquiry-based approach
Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field o...
| Other Authors: | , , |
|---|---|
| Format: | eBook |
| Language: | Inglés |
| Published: |
Warsaw, [Poland] ; Berlin, [Germany] :
De Gruyter
2014
2014. |
| Edition: | 1st ed |
| Subjects: | |
| See on Biblioteca Universitat Ramon Llull: | https://discovery.url.edu/permalink/34CSUC_URL/1im36ta/alma991009427705606719 |
Table of Contents:
- Front matter
- Preface
- Contents
- 1 Introducing the Bacterial Antibiotic Sensor Mini Project
- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
- 3 Designing Primers for Site-Directed Mutagenesis
- 4 Performing Site-Directed Mutagenesis
- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- 11 Understanding the Importance of Buffers in Biological Systems
- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
