Box-Behnken design DNA binding DSC Design of Experiment (DoE) FT-IR NMR PLGA PLGA nanoparticles PLP Technology: general issues cancer therapy chitosan chitosan-PLGA polymer controlled drug delivery controlled release covalent drug conjugation dermal delivery dissolving microneedles disulfide bond finite dose full factorial design glyconanoparticles hybrid polymers hydrogel immunotherapy infectious diseases mannose receptors metronidazole monosodium iodoacetate multiple sclerosis