CFPS DNA origami E. coli crude extract preparation antimicrobials artificial cell bioconversion platform cell-free metabolic engineering cell-free metabolic engineering (CFME) cell-free protein expression (CFPE) cell-free protein synthesis cell-free protein synthesis (CFPS) cell-free synthetic biology cell-free transcription-translation chaperones colicins drug development enzymes eukaryotic lysates genomically engineered E. coli growth factors in vitro protein synthesis in vitro transcription-translation (TX-TL) in vitro translation ischemic stroke mathematical model microsomes protein production rapid prototyping riboregulator sonication